Preparation and characterization of skin tissue engineering scaffolds based on nanometer zinc oxide
Background: Skin is the largest organ of the human body, which has many functions such as protection, sensation and temperature regulation. Skin injury is a common clinical problem that seriously affects patients' quality of life and social economy. Traditional skin repair methods, such as autologous skin transplantation, allograft skin transplantation, artificial skin, etc., have certain limitations, such as donor shortage, rejection reaction, infection risk, high cost and so on. Therefore, it is an important research direction to develop new skin tissue engineering scaffolds to promote skin regeneration and functional recovery.
Project information: The aim of this project is to prepare a skin tissue engineering scaffold based on nanometer zinc oxide (ZnO), and characterize its physical, chemical and biological properties, as well as its effects on skin cells and tissues. Nanometer zinc oxide is a kind of inorganic nanomaterial with various functional properties, such as photocatalysis, antibacterial, anti-inflammatory, antioxidant, stimulating growth factor release, etc. In this project, nano-zinc oxide will be combined with different types of bio-polymers (such as gelatin, collagen, chitosan, etc.), and porous scaffolds with appropriate pore structure and mechanical strength will be prepared by 3D printing technology, and their characterization and evaluation will be conducted.
Project content:
Preparation and characterization of nano-zinc oxide: nano-zinc oxide particles with different morphology and size were prepared by hydrothermal method or sol-gel method, and their morphology, structure, composition and functional properties were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and other methods.
Preparation and characterization of nanoscale zinc oxide and biomolecular composite scaffold: A suitable biomolecular material was selected as the matrix material and mixed with nano-sized zinc oxide particles in a certain proportion to prepare a uniformly dispersed composite ink. Using 3D printing technology, porous scaffolds with different shapes and sizes are printed according to preset parameters and patterns, and post-processing is carried out. The morphology, structure and composition of the scaffolds were characterized by SEM, XRD and FTIR, and the porosity, mechanical properties and degradation properties of the scaffolds were evaluated by water analyzer, universal testing machine and rheometer.
Evaluation of biological properties of nano-zinc oxide and biomolecular composite scaffolds: The effects of scaffolds on skin cells and tissues were evaluated by in vitro and in vivo experiments. In vitro experiments included: (1) Evaluating the antibacterial effect of stents against common skin pathogens (such as staphylococcus aureus, Escherichia coli, etc.); (2) To evaluate the effects of scaffolds on the cytocompatibility, proliferation, migration, differentiation and secretion of skin-related cells such as human keratinocytes (HaCaT), human fibroblasts (HDF), and human epidermal stem cells (hEpSC); (3) The ability of scaffold to bind, penetrate, repair and rebuild skin tissue models (such as skin slices or 3D skin tissue) was evaluated. In vivo experiments included: (1) evaluating the biocompatibility, inflammatory response, degradation behavior and tissue response of the scaffolds after subcutaneous implantation in animal models (such as mice or rabbits); (2) To evaluate the repair effect of stent on full-thickness skin defect or burn wound in animal models, including wound healing rate, neovascularization, new bone formation, scar formation and other indicators.
Topic innovation:
In this study, nano-zinc oxide was first combined with different types of biomolecules, and skin tissue engineering scaffolds with various functional properties were prepared by 3D printing technology, providing a new material platform for skin repair and regeneration.
In this study, nano-zinc oxide photocatalysis, antibacterial, anti-inflammatory, antioxidant and other functions were used to improve the anti-infection ability of the scaffold, reduce the risk of postoperative complications, and promote the proliferation, differentiation and functional recovery of skin cells and tissues.
In this study, the function of stimulating growth factor release of nano-zinc oxide was used to enhance the ability of binding, penetration, repair and reconstruction of the skin tissue model and animal model, and improve the clinical application prospect of the scaffold.
This study systematically characterized and evaluated the physical, chemical and biological properties of scaffolds, as well as their effects on skin cells and tissues, revealed the interaction mechanism between scaffolds and skin, and provided a theoretical basis for the optimal design and improvement of scaffolds.
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