Embolization therapy of tumor based on DNA nanorobots
Background: Cancer is a serious disease that endangers human health. The current therapeutic methods mainly include surgery, chemoradiotherapy and targeted drugs, but they all have certain limitations and side effects. Tumor blood vessels are an important basis for tumor growth and metastasis. By blocking the blood supply of tumor blood vessels, the development of tumor can be effectively inhibited. Thrombin is a key enzyme that can induce thrombus formation. If it can be accurately delivered to tumor blood vessels and released, the purpose of tumor embolization therapy can be realized. However, how to safely transport thrombin to the tumor site and release it at the right time is a huge challenge.
Project introduction: This project aims to construct an intelligent nanorobot using DNA nanotechnology for tumor embolization therapy. The nanorobot is made of DNA self-assembly, has a tubular structure, the internal cavity can be loaded with thrombin as a drug carrier, and the two ends are respectively modified with nuclein aptamer and DNA switch as targeting and response elements. When the nanorobots enter the blood circulation, the nuclein aptamer can recognize and bind to the nuclein receptors on the surface of tumor-related endothelial cells to achieve the targeting of tumor blood vessels. When the nanorobot binds to the nucleolin, the DNA switch will undergo conformational changes, causing the nanorobot to change from a tubular structure to a flat structure, thus releasing the thrombin loaded inside. Thrombin activates the blood clotting system at the tumor site and induces tumor blood vessels to form thrombus, thus achieving the effect of inhibiting tumor growth and metastasis.
Project content:
Design and synthesis of various components of DNA nanorobots, including DNA tubes, nuclein aptamers, DNA switches and thrombin;
Through DNA self-assembly technology, each component is assembled into a complete nanorobot, and it is characterized and optimized.
To evaluate the targeting, responsiveness, stability and safety of nanorobots in vitro and in vivo;
To evaluate the effect of embolization treatment by nanorobots in different types and models of tumors, and explore its mechanism and influencing factors;
Expand the functions and applications of nanorobots, such as their use in combination or in concert with other drugs or therapies.
Topic innovation:
For the first time, DNA nanotechnology was used to construct an intelligent nanorobot for tumor embolization therapy.
Efficient targeting of tumor-associated endothelial cells was achieved by using nucleolin aptamers, and controlled release of thrombin was achieved by using DNA switches.
Effective embolization therapy was realized for various types and models of tumors with good safety and biocompatibility.
It provides a new idea and platform for the design and application of nanomedicine, and a new strategy and means for the treatment of cancer.
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