Project Name:
Development and application of mid-infrared spectroscopy system based on single molecule
Project background:
The enzyme catalyst has the advantages of high catalytic activity and mild catalytic conditions, and can be used to carry out selective catalytic reactions. However, the stability of free enzymes is poor, and its stability can be effectively improved by immobilizing enzymes. Zr-MOF has a high specific surface area and a regulated nanopore structure, which can be used for enzyme immobilization. Polymers have excellent biocompatibility and can be used to modify the surface of inorganic materials to improve the application of materials in biological systems.
Brief introduction of the project:
In this paper, the composite modification of perovskite nanocrystals with Zr-MOF and amphiphilic polymer is used to prepare enzyme biomimetic cascade catalyst. To investigate the effect of amphiphilic polymer on Zr-MOF immobilized enzyme, optimize the composition and structure of the material, and evaluate its catalytic activity, stability and reuse performance.
The goal of this project is to develop a spectral system capable of detecting infrared absorption in a single molecule. The traditional mid-infrared absorption spectrum needs to measure many molecules in the sample, but this project will use vibration-induced fluorescence method to detect the mid-infrared absorption of a single molecule by detecting the modulation of emitted photons by molecular vibration. The advantage of this method is that it can study samples of low concentrations or even single molecules, providing chemical information at the single-molecule level.
Project content:
1. Optimized synthesis of perovskite nanocrystals;
2. Preparation of Zr-MOF nanostructures;
3. Synthesis of amphiphilic polymers;
4. Perovskite modified with Zr-MOF compound amphiphilic polymer;
5. Immobilization of enzymes;
6. Catalyst characterization;
7. Evaluation of catalytic performance.
Topic innovation:
1. Inorganic nanomaterials modified by Zr-MOF and amphiphilic polymer were used for enzyme immobilization;
2. Amphiphilic polymer was added to optimize the microenvironment of Zr-MOF immobilized enzyme and enhance the activity and stability of the enzyme.
Research objectives:
1. Design and prepare enzyme bionic composite nanocatalysts;
2. Evaluate the catalytic activity, selectivity and stability of the material;
3. To investigate the effect of amphiphilic polymer on enzyme immobilization;
4. Provide a new idea for the design of enzyme immobilization catalyst.
The design of this project includes the design and synthesis of materials, structural regulation, performance evaluation and other contents, which not only considers the knowledge of material science, but also integrates certain knowledge of biochemistry and catalysis.